Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 GPR149 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0420C2A)
The PathHunter GPR149 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR149 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR149 and EA-tagged β-Arrestin-2. Activation of GPR149 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS TrkB Functional Assay, 2 vials. (93-0463C3)
The PathHunter U2OS TrkB Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter CHO-K1 THRβ Protein Interaction Cell Line, 2 vials. (93-0391C2)
The PathHunter THRβ Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. triiodothyronine) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged THRβ NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of THRβ, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Eurofins Discoverx PathHunter HEK 293 GPR152 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0384C1A)
The PathHunter GPR152 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR152 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR152 and EA-tagged β-Arrestin-2. Activation of GPR152 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 PPARγ Protein Interaction Cell Line, 2 vials. (93-0390C2)
The PathHunter PPARγ Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. prostaglandin J2, fatty acids) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. The included cell line overexpresses ProLink-tagged PPARγ NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of PPARγ, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
Eurofins Discoverx PathHunter CHO-K1 GPR141 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0385C2A)
The PathHunter GPR141 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR141 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR141 and EA-tagged β-Arrestin-2. Activation of GPR141 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GPR152 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0384C2A)
The PathHunter GPR152 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR152 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR152 and EA-tagged β-Arrestin-2. Activation of GPR152 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 OXTR β-Arrestin Cell Line, 2 vials. (93-0261C2)
The PathHunter OXTR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. oxytocin) based activation of OXTR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXTR and EA-tagged β-Arrestin-2. Activation of OXTR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Cayman Chemical ApelIn-12humnmouserat10mg
An endogenous agonist of the APJ receptor; induces calcium mobilization in APJ-Gαq16 cells expressing human APJ (EC50 = 0.18 nM); inhibits HIV-1 and HIV-2 infection in NP-2/CD4/APJ cells (mean IC50 = 63 UG/ml); inhibits or stimulates the expression of genes associated with apoptosis, mitosis, and DNA repair in IEC-6 and Caco-2 cells, respectively, at 0.01 µM; induces the production of NO in HUVECs from 100-1,000 nM; increases gastric acid secretion, as well as stomach histamine synthesis and secretion in rats from 20-100 UG/kg; reduces MAP in rats at 15 UG/kg
Eurofins Discoverx PathHunter C2C12 KISS1R β-Arrestin Cell Line, 2 vials. (93-0504C7)
The PathHunter KISS1R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. [ligand]) based activation of KISS1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged KISS1R and EA-tagged β-Arrestin-2. Activation of KISS1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 BAI3 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0547C2)
The PathHunter BAI3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of BAI3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BAI3 and EA-tagged β-Arrestin-2. Activation of BAI3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 P2RY6 β-Arrestin Cell Line, 2 vials. (93-0500C2)
The PathHunter P2RY6 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. UTP) based activation of P2RY6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY6 and EA-tagged β-Arrestin-2. Activation of P2RY6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 HRH3 β-Arrestin Cell Line, 2 vials. (93-0509C2)
The PathHunter HRH3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. histamine) based activation of HRH3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH3 and EA-tagged β-Arrestin-2. Activation of HRH3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 HRH1 β-Arrestin Cell Line, 2 vials. (93-0503C2)
The PathHunter HRH1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. histamine) based activation of HRH1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH1 and EA-tagged β-Arrestin-2. Activation of HRH1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mSSTR2 β-Arrestin Cell Line, 2 vials. (93-0616C2)
The PathHunter mSSTR2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. somatostatin 28) based activation of mSSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mSSTR2 and EA-tagged β-Arrestin-2. Activation of mSSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.